8Z20

Crystal structure analysis of thermotolerant Oscillatoria Phycocyanin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	RRCAT INDUS-2 BEAMLINE PX-BL21
Synchrotron site	RRCAT INDUS-2
Beamline	PX-BL21
Temperature [K]	80
Detector technology	CCD
Collection date	2020-11-17
Detector	MARRESEARCH
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	84.012, 153.728, 207.034
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.010 - 2.500
R-factor	0.19976
Rwork	0.197
R-free	0.25964
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.654
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.010	2.540
High resolution limit [Å]	2.500	2.500
Number of reflections	93421	4580
<I/σ(I)>	10.3
Completeness [%]	99.9
Redundancy	6.3
CC(1/2)	0.997	0.701

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2M posttasium formate, 20% (W/V) PEG 3350 at pH 7.3