8Z1U

Crystal structure of aminoglycoside efflux transporter MexY from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-23
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.000
Spacegroup name	P 61 2 2
Unit cell lengths	186.432, 186.432, 374.011
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	161.450 - 2.890
R-factor	0.2539
Rwork	0.253
R-free	0.26990
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.684
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	161.450	2.990
High resolution limit [Å]	2.890	2.890
Number of reflections	81724	4086
<I/σ(I)>	32.1
Completeness [%]	95.9
Redundancy	303
CC(1/2)	0.990	0.618

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1 M Sodium phosphate (pH 6.5), 2.0 - 2.3 M ammonium sulfate