8Z1P
Crystal structure of Saccharomyces cerevisiae isoleucyl-tRNA synthetase in complex with a mimic tRNA(Met) and isoleucine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NFPSS BEAMLINE BL19U1 |
| Synchrotron site | NFPSS |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.662, 81.206, 106.171 |
| Unit cell angles | 78.39, 83.65, 71.78 |
Refinement procedure
| Resolution | 75.920 - 2.830 |
| R-factor | 0.23848 |
| Rwork | 0.237 |
| R-free | 0.27772 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.202 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.920 | 2.990 |
| High resolution limit [Å] | 2.830 | 2.830 |
| Rmerge | 0.049 | 0.239 |
| Rmeas | 0.069 | 0.338 |
| Rpim | 0.049 | 0.239 |
| Total number of observations | 65750 | 9698 |
| Number of reflections | 36523 | 5453 |
| <I/σ(I)> | 10 | 2.5 |
| Completeness [%] | 93.9 | |
| Redundancy | 1.8 | 1.8 |
| CC(1/2) | 0.994 | 0.926 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 281 | 2% TacsimateTM pH 6.0, 0.1 M BIS-TRIS pH 6.5, 20% PEG3350 |
