8Z10
Human beta-catenin crystal structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2016-07-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 63.947, 103.054, 185.883 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.370 - 2.350 |
R-factor | 0.22126 |
Rwork | 0.218 |
R-free | 0.27505 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.013 |
RMSD bond angle | 1.662 |
Data reduction software | HKL-2000 (4.2) |
Data scaling software | HKL-2000 (4.2) |
Phasing software | PHASER (2.0) |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.390 |
High resolution limit [Å] | 2.350 | 2.350 |
Rpim | 0.060 | |
Number of reflections | 26005 | 2467 |
<I/σ(I)> | 9.3 | |
Completeness [%] | 99.3 | |
Redundancy | 2.6 | |
CC(1/2) | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | Apo crystals of beta-Catenin were grown from 14% PEG3350, 0.1M Hepes pH 7.5, 0.2M L-proline. Crystal was transferred into a soaking solution and incubated at RT for 4 days. Crystals were transferred into cryoprotectant (14% PEG3350, 0.1M Hepes pH 7.5, 0.2M L-proline, 20% glycerol), and then flash frozen in liquid nitrogen. |