8Z10

Human beta-catenin crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	298
Detector technology	PIXEL
Collection date	2016-07-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	63.947, 103.054, 185.883
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.370 - 2.350
R-factor	0.22126
Rwork	0.218
R-free	0.27505
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.662
Data reduction software	HKL-2000 (4.2)
Data scaling software	HKL-2000 (4.2)
Phasing software	PHASER (2.0)
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.390
High resolution limit [Å]	2.350	2.350
Rpim		0.060
Number of reflections	26005	2467
<I/σ(I)>	9.3
Completeness [%]	99.3
Redundancy	2.6
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	Apo crystals of beta-Catenin were grown from 14% PEG3350, 0.1M Hepes pH 7.5, 0.2M L-proline. Crystal was transferred into a soaking solution and incubated at RT for 4 days. Crystals were transferred into cryoprotectant (14% PEG3350, 0.1M Hepes pH 7.5, 0.2M L-proline, 20% glycerol), and then flash frozen in liquid nitrogen.