8Z0U
Human beta-catenin crystal structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 298 |
Detector technology | PIXEL |
Collection date | 2016-06-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 63.855, 102.700, 185.923 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 2.210 |
R-factor | 0.22226 |
Rwork | 0.219 |
R-free | 0.28083 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.736 |
Data reduction software | HKL-2000 (4.2) |
Data scaling software | HKL-2000 (4.2) |
Phasing software | PHASER (2.0) |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.250 |
High resolution limit [Å] | 2.210 | 2.210 |
Rpim | 0.070 | |
Number of reflections | 31196 | 2697 |
<I/σ(I)> | 8.2 | |
Completeness [%] | 99.3 | |
Redundancy | 2.9 | |
CC(1/2) | 0.989 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | Apo crystals of beta-Catenin were grown from 14% PEG3350, 0.1M Hepes pH 7.5, 0.2M L-proline. Crystal were transferred into soaking solution and incubated at RT for 4 days. Crystals were transferred into cryoprotectant (14% PEG3350, 0.1M Hepes pH 7.5, 0.2M L-proline, 20% glycerol), and then flash frozen in liquid nitrogen. |