8YZX

Sesquiterpene synthase SydA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17B1
Synchrotron site	SSRF
Beamline	BL17B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-26
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979
Spacegroup name	P 43 2 2
Unit cell lengths	157.928, 157.928, 224.841
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.920 - 3.800
R-factor	0.1818
Rwork	0.179
R-free	0.21190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.681
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.920	3.802
High resolution limit [Å]	3.394	3.394
Rpim	0.064	0.064
Number of reflections	28218
<I/σ(I)>	9
Completeness [%]	98.3
Redundancy	25.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295.15	0.223M magnesium formate, 0.1M Bis-tris pH6.5