8YZQ

Toxascaris leonina galectin (Tl-gal) W77F/W212F oligomer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2023-11-22
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	78.519, 82.154, 114.589
Unit cell angles	90.02, 90.03, 90.01

Refinement procedure

Resolution	28.650 - 2.150
R-factor	0.2367
Rwork	0.234
R-free	0.28630
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.026
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.170
High resolution limit [Å]	2.000	2.150
Rmerge	0.076	0.076
Number of reflections	138948	138948
<I/σ(I)>	14.7	14.7
Completeness [%]	91.2
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	10% polyethylene glycol 8000, 100mm sodium/potassium phosphate (pH 6.2), 200mm NaCl