8YZ3

Crystal structure of Type Six Secretion System adaptor protein Tla3 from Pseudomonas aeruginosa.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER IMUS DIAMOND MICROFOCUS
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-10
Detector	Bruker PHOTON III
Wavelength(s)	1.54
Spacegroup name	C 2 2 21
Unit cell lengths	63.309, 113.001, 160.052
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.254 - 1.800
R-factor	0.1765
Rwork	0.175
R-free	0.20350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.861
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.790	1.850
High resolution limit [Å]	1.790	1.790
Rmerge	0.060	0.648
Rmeas	0.064	0.712
Rpim	0.021	0.288
Number of reflections	447333	29934
<I/σ(I)>	23.4	2.2
Completeness [%]	99.7	97.9
Redundancy	8.2
CC(1/2)	0.999	0.784

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	CHES, PEG 8000, NaCl