8YYK

Structure of RNase J2 wild type at room temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU R-AXIS IV
Temperature [K]	278
Detector technology	IMAGE PLATE
Collection date	2022-10-20
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.541
Spacegroup name	P 1 21 1
Unit cell lengths	65.304, 133.380, 117.751
Unit cell angles	90.00, 92.02, 90.00

Refinement procedure

Resolution	58.600 - 3.200
Rwork	0.205
R-free	0.24770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.118
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0350)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.600	3.370
High resolution limit [Å]	3.200	3.200
Rmerge	0.190	0.600
Number of reflections	29972	4411
<I/σ(I)>	4.6
Completeness [%]	90.1
Redundancy	2.6
CC(1/2)	0.920	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	5.6	291	100mM tri-sodium citrate dihydrate pH 5.6, 20% PEG 4000, 20% Isopropanol, 40% Formamide