8YXX

TRIP4 ASCH domain in complex with a 12bp dsDNA (5'-TGAGGTACCTCG-3')

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-03-30
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 61
Unit cell lengths	66.897, 66.897, 203.101
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.020 - 2.650
R-factor	0.2128
Rwork	0.211
R-free	0.24170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.961
Data scaling software	HKL-2000
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.700
High resolution limit [Å]	2.650	7.190	2.650
Rmerge	0.166	0.063	0.796
Rmeas	0.170	0.065	0.817
Rpim	0.037	0.015	0.183
Total number of observations	306032
Number of reflections	14939	771	754
<I/σ(I)>	3.1
Completeness [%]	100.0	99.5	100
Redundancy	20.5	19.4	19.8
CC(1/2)	0.997	0.996	0.936

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.1 M Succinic acid pH 7.0 0.1 M BICINE pH 8.5 30% v/v Polyethylene glycol monomethyl ether 550