8YXT

Crystal structure of PtmB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-05-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 43 2 2
Unit cell lengths	88.712, 88.712, 139.892
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.970 - 2.600
Rwork	0.227
R-free	0.26130
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.970	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.100
Number of reflections	17583	2136
<I/σ(I)>	26.7
Completeness [%]	98.4
Redundancy	25.5
CC(1/2)		0.912

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1M BIS-Tris pH 5.5 0.2M Ammonium sulfate 25% PEG3350