8YXK
X-ray structure of Clostridioides difficile endolysin Ecd09610 glucosaminidase domain.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2021-07-21 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.430, 80.470, 48.770 |
| Unit cell angles | 90.00, 104.12, 90.00 |
Refinement procedure
| Resolution | 19.322 - 1.870 |
| Rwork | 0.247 |
| R-free | 0.28680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.186 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.322 | 1.920 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 29563 | 2151 |
| <I/σ(I)> | 9 | |
| Completeness [%] | 98.5 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.996 | 0.761 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 293 | 20% (w/v) PEG 3350, 200mM sodium thiocyanate |
