8YX0

Crystal structure of Procambarus clarkii Arginine kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	190
Detector technology	PIXEL
Collection date	2019-11-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	43.740, 59.900, 140.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.528 - 1.566
R-factor	0.1847
Rwork	0.183
R-free	0.21660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.323
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.530	1.600
High resolution limit [Å]	1.560	1.560
Number of reflections	52746	52481
<I/σ(I)>	1.38
Completeness [%]	99.5
Redundancy	5
CC(1/2)	0.970	0.682

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298.15	0.1 M NaCl, 10% Glycerol, 25% PEG-4000, 0.1 M Cacodylate