8YVV

The Crystal Structure of BTK from Biortus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-06-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978530
Spacegroup name	P 21 21 21
Unit cell lengths	46.686, 102.995, 117.543
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.976 - 2.250
Rwork	0.209
R-free	0.26550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.821
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.330	2.320
High resolution limit [Å]	2.250	2.250
Rmerge	0.010	0.970
Number of reflections	27721	2516
<I/σ(I)>	10.9
Completeness [%]	99.9
Redundancy	7.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M LiCl2, 0.1M Tris pH8.0, 20% PEG6000