8YV9

Crystal structure of Caenorhabditis elegans HIM-8 ZF1-2-CTD domain in complex with Chromosome X pairing center

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	59.021, 54.296, 76.770
Unit cell angles	90.00, 109.07, 90.00

Refinement procedure

Resolution	27.890 - 2.140
R-factor	0.1807
Rwork	0.179
R-free	0.22180
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.009
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXCD
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.180
High resolution limit [Å]	2.140	5.810	2.140
Rmerge	0.057	0.040	0.328
Rmeas	0.066	0.046	0.397
Rpim	0.033	0.023	0.220
Total number of observations	50982
Number of reflections	12651	668	531
<I/σ(I)>	13.6
Completeness [%]	98.6	99.6	84.3
Redundancy	4	4	2.7
CC(1/2)	1.000	0.997	0.894

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	25% w/v PEG 3350, 0.1M Bis-Tris pH 5.5, and 0.1 M Ammonium Sulphate