8YV7
X-ray structure of Thialysine N-epsilon-acetyltransferase from Caenorhabditis elegans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2023-05-18 |
| Detector | RDI CMOS_8M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 57.329, 65.098, 65.322 |
| Unit cell angles | 80.62, 72.76, 63.98 |
Refinement procedure
| Resolution | 34.250 - 3.380 |
| R-factor | 0.1865 |
| Rwork | 0.183 |
| R-free | 0.25220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.377 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.250 | 3.490 |
| High resolution limit [Å] | 3.370 | 3.370 |
| Number of reflections | 9322 | 9233 |
| <I/σ(I)> | 5.4 | |
| Completeness [%] | 81.9 | |
| Redundancy | 2.6 | |
| CC(1/2) | 0.919 | 0.771 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 16% w/v polyethylene glycol 8,000;20% v/v glycerol;40mM potasslum dl-hydrogen phosphate |
