8YUK

Structure of a triple-helix region of human collagen type VII

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2024-03-27
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	18.851, 19.248, 78.038
Unit cell angles	90.00, 91.47, 90.00

Refinement procedure

Resolution	26.000 - 1.780
R-factor	0.1979
Rwork	0.196
R-free	0.24170
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.425
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	BALBES
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.000	1.840
High resolution limit [Å]	1.780	1.780
Rmerge		0.097
Number of reflections	5180	311
<I/σ(I)>	17.4
Completeness [%]	91.9
Redundancy	4.2
CC(1/2)	0.998	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2M Ammonium Nitrate, PH 6.3.