8YUD

Crystal structure of Xylose isomerase from Streptomyces avermitilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2022-10-23
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9794
Spacegroup name	P 32 2 1
Unit cell lengths	129.135, 129.135, 233.058
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.210 - 2.810
R-factor	0.1888
Rwork	0.187
R-free	0.24270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.136
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.850
High resolution limit [Å]	2.800	2.800
Number of reflections	54119	2664
<I/σ(I)>	11.24
Completeness [%]	97.8
Redundancy	6.4
CC(1/2)	0.977	0.627

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	Tris, Polyethylene glycol 8,000