8YTP

Single-chain Fv antibody of E11 complex with NP-glycine under reducing conditions

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-10-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.8
Spacegroup name	P 21 21 21
Unit cell lengths	35.680, 61.420, 88.820
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.990 - 1.360
R-factor	0.1473
Rwork	0.146
R-free	0.17480
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6k4z
RMSD bond length	0.012
RMSD bond angle	1.256
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.440
High resolution limit [Å]	1.360	1.360
Rmerge	0.101	1.004
Number of reflections	42209	6460
<I/σ(I)>	10.3	1.4
Completeness [%]	98.5
Redundancy	6.2
CC(1/2)		0.550

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1 M HEPES sodium pH 7.5, 10% v/v 2-propanol, 20% w/v polyethylene glycol 4000