8YTO
Single-chain Fv antibody of E11 complex with NP-glycine
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 33.590, 41.570, 161.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.430 - 1.040 |
| R-factor | 0.1077 |
| Rwork | 0.107 |
| R-free | 0.12360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6k4z |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.285 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.100 |
| High resolution limit [Å] | 1.040 | 1.040 |
| Rmerge | 0.216 | 1.444 |
| Number of reflections | 109128 | 15999 |
| <I/σ(I)> | 23.1 | 8.8 |
| Completeness [%] | 99.1 | 95.2 |
| Redundancy | 70.4 | 54.6 |
| CC(1/2) | 0.727 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES sodium pH 7.5, 10% v/v 2-propanol, 20% w/v polyethylene glycol 4000 |
