8YTH

Crystal structures of human IRF2BP2 RING domain in complex with ZBTB16 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-15
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9792
Spacegroup name	P 64
Unit cell lengths	47.315, 47.315, 57.489
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.370 - 2.400
R-factor	0.2447
Rwork	0.243
R-free	0.28070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.752
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.161	0.159	0.455
Rmeas	0.165	0.165	0.466
Rpim	0.038	0.042	0.103
Total number of observations	58179
Number of reflections	2903	162	123
<I/σ(I)>	5.1
Completeness [%]	100.0	99.4	100
Redundancy	20	16.8	20.6
CC(1/2)	0.912	0.996	0.970

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M potassium thiocyanate and 30% (w/v) PEG MME 2000