8YT5
SP1746 treated with EDTA, in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-28 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 0.984050 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 69.271, 69.271, 88.463 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.010 - 1.600 |
| R-factor | 0.19407 |
| Rwork | 0.194 |
| R-free | 0.20216 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 32997 | 2407 |
| <I/σ(I)> | 31.97 | |
| Completeness [%] | 100.0 | |
| Redundancy | 19.1 | |
| CC(1/2) | 0.999 | 0.843 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 287.2 | 20% PEG3350,0.2 M Potassium sulfate |
