8YSS

Crystal structure of the apo form of Deinococcus wulumuqiensis CD-NTase DwCdnB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-26
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.97857
Spacegroup name	P 1 21 1
Unit cell lengths	35.344, 111.153, 47.120
Unit cell angles	90.00, 100.18, 90.00

Refinement procedure

Resolution	26.770 - 1.830
R-factor	0.1663
Rwork	0.164
R-free	0.19630
Structure solution method	SAD
Data scaling software	HKL-2000
Refinement software	PHENIX (1.17.1-3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.900
High resolution limit [Å]	1.830	1.830
Rmerge	0.071
Number of reflections	30856	3004
<I/σ(I)>	33.225
Completeness [%]	97.9
Redundancy	7.4
CC(1/2)		0.946

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M HEPES pH 7.7, 10 % v/v 2-Propanol, 16 % w/v Polyethylene glycol 4,000.