8YSQ

Crystal structure of SAVED domain of Cap5 from Deinococcus wulumuqiensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 07A
Synchrotron site	NSRRC
Beamline	TPS 07A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-21
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97626
Spacegroup name	P 1
Unit cell lengths	37.468, 43.126, 45.047
Unit cell angles	77.75, 67.35, 74.93

Refinement procedure

Resolution	23.980 - 1.760
R-factor	0.1823
Rwork	0.180
R-free	0.22690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.785
Data scaling software	HKL-2000
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.830
High resolution limit [Å]	1.760	1.760
Rmerge	0.044
Number of reflections	23396	2347
<I/σ(I)>	31.206
Completeness [%]	96.2
Redundancy	5.7
CC(1/2)	0.995	0.992

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M Bis-Tris pH 6.5, 28 % w/v PEG 2000 MME.