8YSN

Crystal structure of the Deinococcus wulumuqiensis CD-NTase DwCdnB Ear-cut mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 07A
Synchrotron site	NSRRC
Beamline	TPS 07A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-14
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97625
Spacegroup name	P 21 21 21
Unit cell lengths	49.344, 73.161, 101.605
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.206 - 2.010
Rwork	0.178
R-free	0.20820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.460
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.080
High resolution limit [Å]	2.010	2.010
Number of reflections	25160	2467
<I/σ(I)>	27.311
Completeness [%]	99.9
Redundancy	12.4
CC(1/2)	0.996	0.904

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1 M Bis-Tris pH 6.5, 0.2 M Sodium fluoride, 20 % w/v PEG 3350.