8YSF

MERS-CoV RBD in complex with nanobody Nb9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97856
Spacegroup name	P 41 21 2
Unit cell lengths	85.819, 85.819, 248.833
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.340 - 2.760
R-factor	0.2347
Rwork	0.233
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.698
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.760	2.760
Number of reflections	24412	2333
<I/σ(I)>	29.6
Completeness [%]	98.3
Redundancy	20
CC(1/2)	0.999	0.836

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	24% PEG1500,100mM SPG, pH 8.4