8YSB

Crystal structure of DynA1, a putative monoxygenase from Mivromonospora chersina.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-08-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	C 2 2 21
Unit cell lengths	33.976, 84.160, 83.228
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.080 - 2.300
R-factor	0.2678
Rwork	0.266
R-free	0.28940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.996
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.090	2.382
High resolution limit [Å]	2.300	2.300
Rmerge	0.107
Number of reflections	5586	533
<I/σ(I)>	12.26
Completeness [%]	99.7
Redundancy	12.6
CC(1/2)	0.998	0.681

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	25% PEG 3350, 8% Tacsimate pH7.5