8YSB
Crystal structure of DynA1, a putative monoxygenase from Mivromonospora chersina.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-08-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 33.976, 84.160, 83.228 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.080 - 2.300 |
| R-factor | 0.2678 |
| Rwork | 0.266 |
| R-free | 0.28940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.996 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.090 | 2.382 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.107 | |
| Number of reflections | 5586 | 533 |
| <I/σ(I)> | 12.26 | |
| Completeness [%] | 99.7 | |
| Redundancy | 12.6 | |
| CC(1/2) | 0.998 | 0.681 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG 3350, 8% Tacsimate pH7.5 |
