8YRI

Crystal structure of sugar phosphotransferase system EIIB component CPF_0401 from Clostridium perfringens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-15
Detector	DECTRIS EIGER R 4M
Wavelength(s)	1.000
Spacegroup name	P 1 21 1
Unit cell lengths	35.253, 69.811, 61.863
Unit cell angles	90.00, 90.25, 90.00

Refinement procedure

Resolution	35.250 - 1.900
R-factor	0.2167
Rwork	0.215
R-free	0.25730
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.264
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.250	2.020
High resolution limit [Å]	1.900	1.900
Rmerge	0.059	0.362
Rmeas	0.073	0.453
Number of reflections	23271	3618
<I/σ(I)>	10.29	2.34
Completeness [%]	98.0	94.9
Redundancy	2.64	2.56
CC(1/2)	0.996	0.864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.3	293	0.2M magnesium chloride, 0.1M MES (pH6.3), 20% w/v PEG 6000