8YRF
Crystal structure of LmrR with V15 replaced by unnatural amino acid 4-amino-L-phenyl-cysteine
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-27 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 32 |
| Unit cell lengths | 63.310, 63.310, 63.920 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.650 - 2.660 |
| R-factor | 0.22942 |
| Rwork | 0.227 |
| R-free | 0.27441 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.252 |
| Data reduction software | ADDREF |
| Data scaling software | SCALA |
| Phasing software | ACORN |
| Refinement software | PHENIX (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.650 | 2.790 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Rmerge | 0.069 | 0.597 |
| Rpim | 0.036 | 0.321 |
| Number of reflections | 8266 | 1104 |
| <I/σ(I)> | 15.7 | |
| Completeness [%] | 99.9 | 99.5 |
| Redundancy | 4.5 | |
| CC(1/2) | 0.997 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.15 | 0.3 M Magnesium nitrate, 0.1 M Tris-HCl, pH 8.0, 20%(w/v) PEG 2000 and 2% (w/v) MPD |
