8YRE

Crystal structure of Arabidopsis VTC1-KJC2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	C 1 2 1
Unit cell lengths	158.268, 134.987, 168.473
Unit cell angles	90.00, 117.14, 90.00

Refinement procedure

Resolution	149.920 - 3.540
R-factor	0.2638
Rwork	0.261
R-free	0.29520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.562
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.18)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	149.920	3.640
High resolution limit [Å]	3.540	3.540
Rmerge	0.139	0.616
Rpim	0.075	0.467
Number of reflections	37074	5264
<I/σ(I)>	7.8	2.4
Completeness [%]	96.4
Redundancy	5
CC(1/2)		0.768

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	ammonium sulphate, Bis-Tris propane, PEG 3350