8YQL

Roadblock domain from a Lokiarchaeota archaeon Roadblock-Longin protein in P31

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.99999
Spacegroup name	P 31
Unit cell lengths	74.816, 74.816, 58.666
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.490 - 2.200
R-factor	0.206
Rwork	0.203
R-free	0.25570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.001
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.490	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.059	0.899
Rmeas	0.073	1.119
Rpim	0.044	0.655
Number of reflections	18642
<I/σ(I)>	7.9	1
Completeness [%]	98.0	99.65
Redundancy	2.64	2.68
CC(1/2)	0.996	0.371

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M sodium chloride, 0.1 M sodium cacodylate, pH 6.5, 1.0 M bi-sodium citrate