8YQK
Roadblock domain from a Lokiarchaeota archaeon Roadblock-Longin protein in P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-12 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.75000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.879, 103.296, 58.179 |
| Unit cell angles | 90.00, 108.21, 90.00 |
Refinement procedure
| Resolution | 19.350 - 1.170 |
| R-factor | 0.185 |
| Rwork | 0.184 |
| R-free | 0.20330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.928 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.700 | 1.210 |
| High resolution limit [Å] | 1.170 | 1.170 |
| Rmerge | 0.042 | 0.779 |
| Rmeas | 0.058 | 1.057 |
| Rpim | 0.040 | 0.710 |
| Number of reflections | 138815 | |
| <I/σ(I)> | 6.5 | 0.7 |
| Completeness [%] | 96.3 | 97.05 |
| Redundancy | 1.8 | 1.73 |
| CC(1/2) | 0.997 | 0.529 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MIB buffer, pH 8.0, 25 % w/v PEG1500 |
