8YQG
Dimeric structure of the Longin domain from a Lokiarchaeota archaeon Roadblock-Longin protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 05A |
Synchrotron site | NSRRC |
Beamline | TPS 05A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-11-02 |
Detector | RAYONIX MX300-HS |
Wavelength(s) | 0.97852 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 76.233, 120.758, 44.807 |
Unit cell angles | 90.00, 104.31, 90.00 |
Refinement procedure
Resolution | 19.680 - 1.770 |
R-factor | 0.1919 |
Rwork | 0.190 |
R-free | 0.22810 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.076 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.680 | 1.830 |
High resolution limit [Å] | 1.770 | 1.770 |
Rmerge | 0.038 | 0.832 |
Rmeas | 0.044 | 0.988 |
Rpim | 0.022 | 0.529 |
Number of reflections | 31416 | 1171 |
<I/σ(I)> | 32.4 | 1.19 |
Completeness [%] | 82.9 | 31 |
Redundancy | 1 | 1 |
CC(1/2) | 0.997 | 0.742 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.2 | 0.2 M sodium citrate, 0.1 M Bis-Tris propane, pH 8.5, 20 % w/v PEG 3350 |