8YQ1

Linear form of FGF10 from Homo sapiens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2020-08-05
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97957
Spacegroup name	P 65 2 2
Unit cell lengths	73.845, 73.845, 227.807
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.920 - 2.560
R-factor	0.2053
Rwork	0.202
R-free	0.27771
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.635
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	REFMAC (5.8.0415)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.920	2.650
High resolution limit [Å]	2.560	2.560
Rmerge	0.062	0.449
Rmeas	0.071
Rpim	0.017
Number of reflections	12263	1143
<I/σ(I)>	18.6
Completeness [%]	97.5
Redundancy	13.5
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	0.2 M Ammonium Sulfate, 10% PEG 4000, 0.1 M Tris-HCl pH 6.5