8YPX

Room temperature structure of TsaGH11 determined by MX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	299
Detector technology	PIXEL
Collection date	2019-12-04
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9794
Spacegroup name	C 2 2 21
Unit cell lengths	100.150, 102.898, 174.636
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.350 - 2.700
R-factor	0.1801
Rwork	0.176
R-free	0.22780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.898
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Number of reflections	24819	1191
<I/σ(I)>	9.54
Completeness [%]	98.2
Redundancy	5.6
CC(1/2)	0.984	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295.15	sodium acetate, ammonium acetate