8YPJ
Cyrstal structure of the MazE-mt10 Antitoxin from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-10-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97959 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 99.370, 99.370, 99.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.910 |
| R-factor | 0.2022 |
| Rwork | 0.200 |
| R-free | 0.24090 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.880 |
| Data reduction software | HKL-2000 (712) |
| Data scaling software | HKL-2000 (712) |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.940 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.097 | 0.097 |
| Number of reflections | 12759 | 648 |
| <I/σ(I)> | 21.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES (pH 6), 20% (w/v) PEG 6000, 0.2 M NH4Cl |
