8YP0

Crystal Structure of Human Rab23 Y79del in Complex with GMPPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-X
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-08
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	40.747, 61.710, 62.189
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.830 - 1.880
R-factor	0.1895
Rwork	0.187
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.696
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.000
High resolution limit [Å]	1.880	5.610	1.880
Rmerge	0.112	0.043	1.120
Rmeas	0.122	0.047	1.220
Number of reflections	13115	557	2033
<I/σ(I)>	12.64
Completeness [%]	99.3	99.1	97.2
Redundancy	6.4
CC(1/2)	0.998	0.999	0.655

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.1M sodium acetate pH 5.0, 0.2M NaCl, 25% PEG 6000