8YOZ
Crystal structure of the small zinc-finger protein ZifS (TTHA0897) from Thermus thermophilus HB8.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-03 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.120, 67.010, 81.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.960 - 1.830 |
| R-factor | 0.2343 |
| Rwork | 0.233 |
| R-free | 0.26360 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.891 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.960 | 1.895 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmeas | 0.064 | 0.465 |
| Number of reflections | 15281 | 1500 |
| <I/σ(I)> | 12.27 | 3.23 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 19.2 | |
| CC(1/2) | 1.000 | 0.962 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 400 mM Sodium phosphate monobasic/ 1600 mM Potassium phosphate dibasic, 100 mM Imidazole/Hydrochloric acid pH 8.0, 200 mM Sodium chloride |
