8YOZ

Crystal structure of the small zinc-finger protein ZifS (TTHA0897) from Thermus thermophilus HB8.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-03
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	30.120, 67.010, 81.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.960 - 1.830
R-factor	0.2343
Rwork	0.233
R-free	0.26360
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.891
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.960	1.895
High resolution limit [Å]	1.830	1.830
Rmeas	0.064	0.465
Number of reflections	15281	1500
<I/σ(I)>	12.27	3.23
Completeness [%]	100.0	100
Redundancy	19.2
CC(1/2)	1.000	0.962

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	400 mM Sodium phosphate monobasic/ 1600 mM Potassium phosphate dibasic, 100 mM Imidazole/Hydrochloric acid pH 8.0, 200 mM Sodium chloride