8YOA

Crystal structure of endolytic transglycosylase MltG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	125
Detector technology	PIXEL
Collection date	2022-07-07
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	43.220, 63.240, 123.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.820 - 2.200
R-factor	0.1834
Rwork	0.181
R-free	0.21930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.867
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.820	2.279
High resolution limit [Å]	2.200	2.200
Rmerge	0.245
Number of reflections	17774	1735
<I/σ(I)>	16.51
Completeness [%]	99.4
Redundancy	12.5
CC(1/2)		0.919

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.9M Sodium Citrate, 0.1M Tris pH 7.4, 0.2M NaCl, 40% (V/V) Acetone