8YO6
Crystal structure of CagT from Helicobacter pylori
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2021-11-19 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 51.090, 86.250, 105.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.050 - 1.950 |
| R-factor | 0.1937 |
| Rwork | 0.192 |
| R-free | 0.22850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.866 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.830 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.079 | 0.464 |
| Number of reflections | 17417 | 1210 |
| <I/σ(I)> | 7.6 | 2.2 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.995 | 0.684 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | Morpheus III F1 condition 0.6% antibiotics mix, 0.1M buffer system 1, 30% v/v precipitant mix 1, 1% 1,4-dioxane |
