8YO0
Crystal Structure of Human Rab23 Y79del in Complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-01-19 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.787, 51.606, 74.597 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.640 - 1.300 |
| R-factor | 0.1595 |
| Rwork | 0.158 |
| R-free | 0.18580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.126 |
| Data reduction software | CrysalisPro |
| Data scaling software | CrysalisPro |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.640 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.086 | 1.025 |
| Rmeas | 0.094 | 1.153 |
| Rpim | 0.038 | 0.519 |
| Total number of observations | 225700 | 9419 |
| Number of reflections | 39490 | 1954 |
| <I/σ(I)> | 11.2 | 1.9 |
| Completeness [%] | 99.9 | |
| Redundancy | 5.7 | 4.8 |
| CC(1/2) | 0.999 | 0.593 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1M SPG pH 5.0, 27.5% PEG 1500 |
