8YNV
Poly(3-hydroxybutyrate) depolymerase PhaZ from Bacillus thuringiensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-22 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.965, 83.227, 85.501 |
| Unit cell angles | 73.44, 82.83, 83.49 |
Refinement procedure
| Resolution | 28.400 - 1.420 |
| R-factor | 0.161 |
| Rwork | 0.161 |
| R-free | 0.18920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1a88 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.752 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.470 |
| High resolution limit [Å] | 1.420 | 1.420 |
| Rmerge | 0.064 | 0.510 |
| Number of reflections | 200742 | 19495 |
| <I/σ(I)> | 21.2 | |
| Completeness [%] | 94.5 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 25% polyethylene glycol 3350, 200 mM ammonium acetate, and 100 mM bis-tris (pH 5.6) |
