8YNU
Crystal structure of the myb domain of S.pombe Tbf1 in the P222 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97778 |
| Spacegroup name | P 2 2 2 |
| Unit cell lengths | 41.393, 62.230, 86.773 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.390 - 1.760 |
| R-factor | 0.1955 |
| Rwork | 0.194 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.886 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.107 | 0.380 |
| Rmeas | 0.112 | 0.396 |
| Rpim | 0.031 | 0.111 |
| Number of reflections | 292977 | 1114 |
| <I/σ(I)> | 36.28 | 6.083 |
| Completeness [%] | 100.0 | |
| Redundancy | 12.9 | |
| CC(1/2) | 0.937 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277.15 | 16% PEG 8000, 40mM Potassium phosphate dibasic, 20% Glycerol |
