8YNP

RNA duplex containing Methylformamide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.91962
Spacegroup name	P 1
Unit cell lengths	25.604, 27.932, 28.668
Unit cell angles	110.03, 114.81, 95.76

Refinement procedure

Resolution	25.150 - 1.400
R-factor	0.1537
Rwork	0.151
R-free	0.17250
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.150	25.150	1.430
High resolution limit [Å]	1.400	6.250	1.400
Rmerge	0.071	0.053	0.231
Rmeas	0.084	0.063	0.270
Total number of observations	43501
Number of reflections	12164	148	841
<I/σ(I)>	12.58	17.53	7.07
Completeness [%]	93.9	96.7	89.3
Redundancy	3.576	3.345	3.711
CC(1/2)	0.993	0.994	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	MPD, sodium cacodylate, spermine tetrahydrochloride