8YNO

RNA duplex containing Formamide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.91962
Spacegroup name	P 1 21 1
Unit cell lengths	27.633, 43.063, 28.043
Unit cell angles	90.00, 109.51, 90.00

Refinement procedure

Resolution	26.430 - 1.300
R-factor	0.1529
Rwork	0.151
R-free	0.17270
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	26.430	26.430	1.330
High resolution limit [Å]	1.300	5.810	1.300
Rmerge	0.059	0.055	0.146
Rmeas	0.064	0.060	0.163
Total number of observations	97678
Number of reflections	15146	181	968
<I/σ(I)>	19.46	26.55	9.51
Completeness [%]	98.3	98.4	84.2
Redundancy	6.449	5.829	5.121
CC(1/2)	0.998	0.997	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	MPD, sodium cacodylate