8YNJ

Crystal structure of Langat virus helicase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2022-08-27
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97915
Spacegroup name	P 41 21 2
Unit cell lengths	68.018, 68.018, 213.125
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.120 - 2.480
R-factor	0.2404
Rwork	0.238
R-free	0.28020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.380
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.130	2.610
High resolution limit [Å]	2.480	2.480
Number of reflections	18766	2670
<I/σ(I)>	11.8
Completeness [%]	100.0
Redundancy	20
CC(1/2)	0.990	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	0.2M Sodium malonate pH 7.0, 17% w/v Polyethylene glycol 3350