8YMA
CRYSTAL STRUCTURE OF A NOVEL PU PLASTIC DEGRADATION ENZYME FROM THERMAEROBACTER MARIANENSIS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 61 |
| Unit cell lengths | 81.834, 81.834, 667.371 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.590 - 2.070 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.22742 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.510 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.590 | 2.100 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Number of reflections | 153365 | 7533 |
| <I/σ(I)> | 11.8 | |
| Completeness [%] | 99.9 | |
| Redundancy | 21.5 | 21.3 |
| CC(1/2) | 0.995 | 0.643 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 1.0M (NH4)2HPO4, 0.1M imidazole pH 8.0, 0.2M NaCl |
