8YLM

Crystal structure of Deinococcus radiodurans HucR in complex with uric acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2022-07-22
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 1
Unit cell lengths	56.815, 57.206, 60.511
Unit cell angles	67.26, 80.25, 79.90

Refinement procedure

Resolution	29.377 - 1.950
R-factor	0.1926
Rwork	0.191
R-free	0.22860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8ylf
RMSD bond length	0.006
RMSD bond angle	0.886
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.15.2_3472: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.980
High resolution limit [Å]	1.950	1.950
Number of reflections	48572	2393
<I/σ(I)>	17.6	2.3
Completeness [%]	97.1	95.9
Redundancy	2	2
CC(1/2)	0.996	0.623

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	PEG 1000, Tris