8YLK

Crystal structure of Burkholderia thailandensis MftR in complex with uric acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2021-07-01
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9793
Spacegroup name	P 31 2 1
Unit cell lengths	62.942, 62.942, 77.568
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.250 - 2.100
R-factor	0.2286
Rwork	0.227
R-free	0.26060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8ylf
RMSD bond length	0.003
RMSD bond angle	0.571
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.057	0.489
Number of reflections	10766	507
<I/σ(I)>	31.2	2.4
Completeness [%]	99.7	98.6
Redundancy	5.1	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	PEG 3350