8YLI
Crystal structure of Pectobacterium atrosepticum PecS in complex with operator DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2022-10-07 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.170, 80.504, 75.233 |
| Unit cell angles | 90.00, 93.48, 90.00 |
Refinement procedure
| Resolution | 27.460 - 2.900 |
| R-factor | 0.2278 |
| Rwork | 0.225 |
| R-free | 0.27430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8ylg |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.840 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Number of reflections | 18873 | 911 |
| <I/σ(I)> | 17.3 | 2.1 |
| Completeness [%] | 99.2 | 99.8 |
| Redundancy | 3 | 3.1 |
| CC(1/2) | 0.992 | 0.789 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291.15 | sodium formate, sodium acetate |
